碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 95 / Abstract
The cell model of neurotoxicity set up by PC12 cells. The instantaneous drop in the fluorescence responses after added Pb2+ was decreased immediately. This result indicated the contribution of fluorescence quench in PC12 cells under different concentration of Ca2+ and Pb2+. It’s showed the cellular uptake of Pb2+ in real-time. This increased of Indo-1 fluorescence quench with increasing the concentration of Pb2+ occurred in both the extracellular normal Ca2+ and Ca2+ free medium but was most apparent while 20μM Pb2+ was added to PC12 cells in the Ca2+ free medium.
In this experiment of the effects on the calcium reaction in PC12 cells by Pb2+. Added ATP（200μM）as a stimulates drug for activates the Ca2+-ATPase. The concentration of calcium reaction in PC12 cell was being inhibited and three times lower than the control（2756.56±40 nM）by obviously, while the Pb2+ added.
The digested cell from the gill , muscle , liver , spleen and bowel of Mugil cephalus fry（5.04±0.12 cm of average long, 2.44±0.15 g of average weight）to be experimental subjects. The instantaneous drop in fluorescence after the addition of Pb2+ was always present and indicated the contribution of fluorescence in all organs cells quench under different concentration of Ca2+ by Pb2+. Show that cellular uptake of Pb2+ in real-time.
The maximum of Indo-1 fluorescence quench in cells of gill , muscle , liver , spleen and bowel were 41.67±0.23%, 23.80±0.43% , 49.51±0.80% , 40.23±0.63% and 37.66±0.57, respectively. Effects of different contribution of Pb2+ under extracellular different Ca2+ contribution medium, the maximum of Indo-1 fluorescence quench was the liver cell, secondly was spleen and intestinal cell, the least was the muscle cell.
Key words: Lead, Grey mullet（Mugil cephalus）, Indo-1.
| Identifer | oai:union.ndltd.org:TW/095NTOU5451009 |
| Date | January 2007 |
| Creators | Yi-Jun Lin, 林怡君 |
| Contributors | Sha-Yen Cheng, 鄭學淵 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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