碩士 / 臺灣大學 / 動物學研究研究所 / 95 / Nervous necrosis viruses (NNV), belonging to fish nodavirus, have caused mass mortality of grouper larvae. Fish iridoviruses, including grouper iridovirus of Taiwan (TGIV) and red sea bream iridovirus (RSIV), are important pathogens for the continuous death of grouper juveniles and adult fish. One aim of the present study is to develop rapid and sensitive nucleic acid-based diagnosis methods for fish nodavirus and two kinds of fish iridoviruses (TGIV, RSIV). Another aim of this study was to screen NNV in wild and cultured fish collected from different aquatic areas and seasons. We successfully established six NNV-specific primers and reaction program for loop-mediated isothermal amplification assay (LAMP), and the proper concentration of primers and chemicals for simultaneous amplification of nucleic acids of TGIV and RSIV in Multiplex PCR. By RT-PCR and nested-PCR screening of NNV, 30 of 75 species of wild fish collected from four season and different coasts of Taiwan were NNV carrier, especially the wild fish from southern area. Mass mortality of cultured grouper larvae by NNV infection occurred since late spring to the end of summer. However, NNV was only detected in the mullets collected during autumn and winter.
| Identifer | oai:union.ndltd.org:TW/095NTU05312029 |
| Date | January 2007 |
| Creators | Kuan-Jung Shao, 邵冠榮 |
| Contributors | 齊肖琪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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