碩士 / 靜宜大學 / 食品營養研究所 / 95 / Cancer is the major death cause in Taiwan. Recently, there is an increase interest in the study how to prevention or delay the hepatoma development. Chlorella has been reported to show antioxidant activity and anticancer functions. In this study, we will examine the antioxidant activities of Chlorella and possible apoptosis mechanim in human hepatoma cell lines (Hep G2 cells). The results demonstrated that 80% ethanolic extracts from commercial granule chlorella products (GPE) and ball milling chlorella products (BME) had α,α-diphenyl-β-picryl hydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfomic acid) (ABTS) radical-scavenging activity and this activity is a dose-dependent manner. GPE and BME can decrease the percentage of viable cells in time- and dose- dependent manners. The morphology, DAPI staining, comet assay were conducted and they indicated the GPE induced Hep G2 cells apoptosis. Flow cytometry assay demonstrated that GPE induce the change of mitochondrial membrane potential, reactive oxygen species (ROS), and calcium releasing. Western blotting analysis, GPE decreased the protein expression of Bcl-2, and increased the levels of cytochrome c. Furthermore, GPE can increase the expression of caspase-9, -3, -8, and also increased the expression of Fas and FasL. In MAPK family, GPE can decrease the expression levels of ERK and increased the levels of JNK. GPE also can inhibit the invasion of Hep G2 cells by suppressing MMP-2, and MMP-9 expression. In addition, GPE decreased the superoxide dismutase activity and glutathione level, however, the GPE increased malondialdehyde, glutathione peroxidase and catalase activity in Hep G2 cells.
The in-vivo antioxidant effects of GPE on carbon tetrachloride (CCl4)-induce oxidative stress in Sprague-Dawley rats. The results showed that the GPE treatment reduced significantly the CCl4-induced increase in serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), inhibited the CCl4-induced lipid peroxidation, increased vitamin C content, SOD and catalase activity, also keep vitamin E and GSH content in liver. Collectively, these results support that GPE can inhibit cells growth, induce apoptosis and inhibit invasiveness in Hep G2 cells. Oral administration with GPE for 28 consecutive days could significant decrease the CCl4-induced hepatic damage by increasing the activity of antioxidant enzymes. The results suggest GPE may have the anticancer potential and antioxidant activity.
| Identifer | oai:union.ndltd.org:TW/095PU005255027 |
| Date | January 1900 |
| Creators | Yu-Chan Chu, 朱育嬋 |
| Contributors | Su-Tze Chou, 周淑姿 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 136 |
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