碩士 / 東吳大學 / 微生物學系 / 95 / According to the previous researches, phthalate esters (PAEs) belong to one group of
endocrine disrupter chemicals (EDCs) for many animals (including human being). They can
be accumulated in the body through the food chain, and further interfere the physiology and
reproduction of both the organism and its offspring. Consequently, to evaluate the PAE
pollution level in the environment and its toxicity is very important. In this study, the
evaluation of Neocaridina denticulate was carried out to test the feasibility of organism
indicator by using one of PAEs, dipropyl phthalate (DPrP).
The results of the effect of DPrP on shrimp immunity showed that the three activities of
superoxide dismutase (SOD), β-Glucuronidase (β-Glu), α-Naphthyl acetate esterase
(ANAE) and the mRNA level of prophenoloxidase (proPO) and transglutaminase (TGase)
significantly increased (p<0.05) after shrimps were bathed in 1 ppm of DPrP for one or
three days. As for the PO activity of shrimp, it was detectable to increase after shrimps were
bathed in 50 ppm of DPrP for one day; however, it was not different from that of the control
after treatment for more than three days.
The result of the effect of DPrP on reproduction of shrimp treated with different dosage
of DPrP was found that the concentration of 17β-estradiol (E2) in the tissue of the 5
ppm-DPrP-treated group significantly increased, and that vitellogenin (Vg) significantly
increased when the dosage of DPrP was 10 ppm.
By analyzing all the results from both the immunity and the reproductivity of
Neocaridina denticulat, the expressions of several biological indicators, including activity
of SOD, β-Glu and AcP of haemocytes, and concentration of Vg and expression of proPO
mRNA of tissues, were dependent on the dosage of DPrP and the length of treatment time
in the process. In addition, the minimal dosage of DPrP required to cause a change of the
biological expression was 1 ppm for indicators of SOD, β-Glu, AcP, and proPO gene, and
10 ppm for Vg. As for the remainders, including the ANAE activity, the E2 concentration
in tissue and the expression of TGase gene, their expression is correlation with neither the
dosage of DPrP nor the length of treatment time. Moreover, PO activity is not a sensitivebiological indicator because 50 ppm of DPrP is required to cause the change of PO activity.
As a result, these indicators are unable to be used in developing a model of expressingbiological indicator.
| Identifer | oai:union.ndltd.org:TW/095SCU05381008 |
| Date | January 2007 |
| Creators | Yi-Hsiu Lin1, 林怡秀 |
| Contributors | H H sung, 宋宏紅 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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