碩士 / 大仁科技大學 / 環境管理研究所 / 95 / The plentiful proliferation of algae in eutrophic lake can result in some problems in the source water, including (1)the severe variation of dissolved oxygen between day and night；(2)the release of algal toxin；(3) the precursor increase of trihalomethane formation potential (THMFP)；(4) the formation of flavor, odor, and color. All above problems lead to the operational difficulty in watertreatment plant. For past several decades, most of researches were focused in the algal removal by various operation units in watertreatment. The improvement for the instrument of monitoring algae, especially in fluorescent spectrometer, was increasingly discussed in foreign researchers. However, few papers were still seldom mentioned in domestic reports. Fluorescent spectrometer (FS) also can be used to characterize the organic properties in source water. It seems possible using fluorescent spectrometer to classify algae and its excrete owing to its superiorities with non-destructive and slight amount of water sample, and quick analysis. The differences of pigments were measured by the tool of high performance liquid chromatography (HPLC).
In this research, the differences of algal species in EEFM (excitation–emission fluorescent matrix) of FS and pigments measured by HPLC were compared for possibly providing more information to waterteatment for adequately operating processes. Three algal species, Chodatella sp., Chlorella sp. and Navicula sp. were selected to conduct all experiments. Four goals also were scheduled as the following statement:
1. To compare the differences of the algae number counted using fluorescence combined with image analysis software, optical microscope with membrane filter and optical microscope with hemocytometer.
2. To establish the EEFM for Chodatella sp., Chlorella sp. and Navicula sp.
3. To characterize the characteristics of organic matter, and to measure the trihalomethane formation potential (THMFP) released from three algal species.
4. To distinguish the major pigments for Chodatella sp., Chlorella sp. and Navicula sp.
The data obtained from three methods for counting algal number have insignificant difference by one way analysis of variance with the 0.083 for F value and above 0.01 for P value. The wavelengths of maximum absorption peak for Chodatella sp., Chlorella sp. and Navicula sp. were respectively found at 684、683 and 680 nm.
During incubation, three algae themselves had same peak location at emission wave length of 280-290 nm and excitation wave length of 330-360 nm. This peak may be protein-like organic matter induced. Two peaks coincidentally appeared at 440/670 nm and 530/670 nm, which belonged to pigments, for both Chodatella sp. and Chlorella sp. in the log phase. The fluorescent intensities of two peaks location for Chodatella sp. were higher than those for Chlorella sp. However, the peak locations for Navicula sp. had not found until the steady state of incubation. The filtrates of three algal waters through 0.2 �慆 membrane filter were analyzed by EEFM of FS. The peaks at 270-290/310-340 nm (protein-like substance), 340-360/400-450 nm (humic-like) and 250-260/430-460 nm (humic-like) can be found for Chodatella sp. and Navicula sp. in whole incubation periods, but only one peak of 340-360/420-450 nm (humic-like) existed in Choralla sp..
Regarding to characteristic of organic matter of filtrates from three algal species, the percentage of carbon content of hydrophobic neutral (HPON) in whole incubation periods was the largest part compared with other organic properties using SFS (spectral fluorescent significature) model. Both hydrophobic base (HPOB) and hydrophilic neutral (HPIN) were not found for all filtrates from three algal species. The percentage of carbon content of hydrophobic acid (HPOA) for Chdatella sp. was superior to that for Chlorella sp, but that for the former both algal filtrate were larger than that for Navicula sp. The THMFP value for filtrate form Chlorella sp. water was the largest, and THMFP of algal water was mainly attributed from the filtrate. Based the ratio of pigments to Chl a., Chlorophyll c2 and Lutein/Zeaxanthin was dominant pigments for Chodatella sp., Chlorophyll b and Lutein/Zeaxanthin for Chlorella sp., and Chlorophyll c2 and Fucoxanthin for Navicula sp.
| Identifer | oai:union.ndltd.org:TW/095TAJ05700009 |
| Date | January 2007 |
| Creators | Yan-hong Chen, 陳彥宏 |
| Contributors | Wen-Liang Lai, Chun-Yen Chiu, 賴文亮, 邱俊彥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 98 |
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