碩士 / 慈濟大學 / 人類遺傳研究所 / 95 / Trace fear conditioning (TFC), a type of hippocampal-dependent learning, is consisted of a conditional stimulus (tone) followed by an unconditional stimulus (foot shock) separated by a period of temporal gap. Retrieval of tone memory for TFC is dependent on the hippocampus. This classical conditioning paradigm is used in our research to investigate the molecular mechanism underlying temporal associative fear learning. In order to identify immediately early genes participating in trace fear learning, we used cDNA microarrays to screen for a total of ~32000 genes from the right and left hippocampus, respectively, of C57BL/6 mouse. Compared mice of conditioned group with that of naïve group, we have already screened a string of genes that were highly expressed in trace fear conditioned mice. Quantitative-PCR was used to further confirm expression levels of candidate genes based on microarray analysis. We chose the gene, vomeronasal type 1 receptor(V1rc), as the first candidate gene and western blotting was employed to quantify the V1rc protein level in 0hr, 3.5hr, 7hr, 12hr after training. Besides, we also applied immunohistochemistry to localized the V1rc expression sites within the hippocampus to deduce the role of V1rc in fear learning and memory. By doing so, we will be able to identify a gene expression profile specific for trace fear conditioning and contribute valuable gene information in the mechanism of learning and memory.
| Identifer | oai:union.ndltd.org:TW/095TCU05498001 |
| Creators | Hung-sheng Hsia, 夏宏昇 |
| Contributors | Yi-chun Liu, 劉怡均 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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