碩士 / 慈濟大學 / 人類遺傳研究所 / 95 / Voltage-dependent calcium channels trigger many intracellular
biochemical events, including muscle contraction, gene expression and
secretion of hormones and neurotransmitters. Of all the voltage-gated
calcium channels, the 1H T-type Ca2+ channel (Cav3.2) is highly
expressed in the hippocampus, where is correlated with contextual
learning and spatial memory. Therefore, we hypothesize that Cav3.2
plays a critical role in hippocampal-dependent learning. In order to
investigate the functional role of the 1H channel in learning and
memory, we performed hippocampal-dependent behavior tests
including trace fear conditioning and passive avoidance task on 1H
homozygous knock-out mice, 1H heterozygous knockout mice and
wild type mice. Results showed that 1H-/- mice are impaired in
contextual fear learning, step-down and step-through tasks.
Subsequently, we infused T type Ca2+ channel blockers (mibefradil
and ethosuximide) to locally knock down the function of 1H T-type
Ca2+ channel. Results showed that mice received blockers displaying
deficits in contextual fear learning. The data indicate that 1H
influences hippocampal-dependent learning via the central nervous
system directly.
| Identifer | oai:union.ndltd.org:TW/095TCU05498007 |
| Creators | Jhe-Wei Shen, 沈哲緯 |
| Contributors | Ingrid Y.-C. Liu, 劉怡均 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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