碩士 / 淡江大學 / 化學學系碩士班 / 95 / 1. A rapid and dynamic liquid phase microextraction technique named Rotating hollow fiber - liquid phase microextraction (RHF-LPME) was developed to couple to GC/MS using electronic ionization (EI) under selective ion monitor (SIM) for quantitative analysis and using self-ion/molecule reaction (SIMR) in conjunction with tandem mass spectrometry for discrimination of five aromatic hydrocarbon isomers including cumene, propylbenzene, 2-ethyltoluene, 1,2,3-trimethylbenzene and 1,2,4-trimethylbenzene.
The optimized parameters of this approach was: organic solvent toluene, extraction time 2min, stirring rate of stirrer 700rpm, rotating speed of motor driving rotator 250rpm, no addition of salt, pH of aqueous phase 6 and both rotator and stirrer were operated in reversed directions. The linear range of calibration curve of RHF-LPME was from 0.002 to 0.4 μg/mL; the R2 was 0.99 and the relative standard deviation (RSD) values were from 4.5 to 5.2%. Comparing to single drop microextraction (SDME), the RHF-LPME method improved the limit of detection (LOD) and enrichment factor (EF) more than one fold. The LODs of RHF-LPME (4-5 pg/mL) were closed to those of (2-3 pg/mL) solid phase microextraction (SPME) but SPME was seriously limited to high cost and peaks broaden.
In addition, comparing to traditional dynamic liquid phase microextraction, this approach with the advantages of easy to operate, high reproducibility, extremely fast, high extraction efficiency and good reliability for analyzing biological samples.
2. A rapid, simple and efficient pharmacokinetic approach for the quantitative determination of trace amount of quinidine drug in fish has been demonstrated by combining single drop microextraction with AP-MALDI/Ion Trap Mass Spectrometry. This technique provide good correlation coefficient (>0.99) in work. The limits of detection of quinidine in water, and fish samples were 0.05 and 0.08μM, respectively. The intraday and interday precision of water and fish samples with relative standard deviations ranging from 6.7% to 9.4% and 8.8% to 9.6%. Results also showed high relative recovery (>91.8%) of fish sample analysis in water. This method of combing SDME with AP-MALDI-MS analysis has proven to be a fast technique for direct preconcention of fish tissues.
| Identifer | oai:union.ndltd.org:TW/095TKU05065009 |
| Date | January 2007 |
| Creators | Yuan-Chin Chen, 陳沅情 |
| Contributors | Hui-Fen Wu, Hsiu-Fu Hsu, 吳慧芬, 徐秀福 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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