碩士 / 淡江大學 / 生命科學研究所碩士班 / 95 / Caffeine is a commonly existing in many kinds of food, including coffee, tea, and coca. However, little is known about the caffeine-induced toxicity and teratogenicity during early embryonic development. Here, we used zebrafish as a model to test the toxicity and teratogenicity because of their transparent eggs; therefore, the organogenesis of zebrafish embryos is easy to observe. After low dosages of caffeine (1-100 ppm) treatment, the zebrafish embryos exhibited a 100 % survival rate. As the exposure dosages increased, the survival rates decreased. No embryos survived after treatment with 1000 ppm caffeine. The 50% lethal dose (LD50) of zebrafish is found to be in the range of 200-275 ppm. Shortened and curved body were observed after treatment with caffeine. Compared to untreated littermates (vehicle-treated control), caffeine-treated embryos exhibited significantly reduced tactile sensitivity frequencies of touch-induced movement (vehicle-treated control: 10±3 v.s. 150 ppm caffeine: 0±0; N=30). Subtle changes are easily observed morphology by staining with specific monoclonal antibodies F59, Znp1 and aat to detect changes in muscle fibers, motor axons, sensory neurons, Rb cell and brain, respectively. Our data showed that the treatment of caffeine led to misalignment of muscle fibers, motor neuron innervations, less acetyl-choline receptor cluster and Rohon-beard cell. On the basis of these observations, we suggest that caffeiene is able to induce neurotoxicity and nephrotoxicity of zebrafish larvae.
| Identifer | oai:union.ndltd.org:TW/095TKU05105015 |
| Date | January 2007 |
| Creators | Yi-Hui Huang, 黃奕惠 |
| Contributors | Yau-Hung Chen, 陳曜鴻 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 73 |
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