碩士 / 臺北醫學大學 / 生藥學研究所 / 95 / 英文摘要
In this study, 250 strains of marine bacteria isolated from Taiwan were cultured for the screening of their antibacterial activities by disc agar diffusion method. Five pathogenic bacteria including Vibrio vulnificus, (ATTC 27562), V. parahaemolyticus, (ATTC 17802), Listonella anguillarum, (ATTC 19264), Staphylococcus aureus, (ATTC 12600), and Aeromonas hydrophila, (ATTC 7966) were tested. Of these bacterial strains monitored, 12 strains exhibited antibacterial activities. Among them, bacterial strains BGN6 (Pseudoalteromonas flavipulchra) and B0 (P. flavipulchra) showed significant antibacterial activity toward V. vulnificus, while BTN3 (V. natriegens) showed significant antibacterial activity specifically toward S. aureus as evidenced from the observation of significant inhibitory zones. Based on these findings, the 3 bioactive bacterial strains were mass cultured using PY medium. Then, the bioactive constituents were obtained by a series of chromatographic separation, and characterized by spectral analysis. Totally 15 compounds including 2 aliphatic alkaloids, 2 pyrimidines, 2 phenolics, 2 indole alkaloids, and 7 diketopiperazines were isolated and identified. Their structures were elucidated to be korormicin (1), uracil (2), 4-(hydroxymethyl)phenol (3), cyclo-L-Pro-L-Phe (4), norkorormicin (5), p-hydroxybenzaldehyde (6), thymine (7), cyclo-L-Pro-L-Val (8), cyclo-L-Pro-L-Met (9), cyclo-L-Pro-L-Ile (10), cyclo-L-Pro-L-Leu (11), cyclo-D-Pro-L-Phe (12), cyclo-L-Pro-L-Tyr (13), indole (14), and 3-(3,3-diindoly)propane-1,2-diol (15). In addition, results of biological tests indicated that 1 and 5 exhibited strong antibacterial activity toward V. vulnificus, and both their minimun inhibitory concentrations were 1 mg/mL. The cytotoxicity tests revealed that 1, 4 and 12 exerted moderate cytotoxic activities against FaDu、MT-2 and NPC-tw01 cancer cell lines when compared with the chemotherapeutic agent, doxorubixin, with an IC50 value of 2.4 ~ 25.3 mM.
Identifer | oai:union.ndltd.org:TW/095TMC05553006 |
Date | January 2007 |
Creators | Li-Wen Lee, 李麗雯 |
Contributors | 李宗徽 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 173 |
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