碩士 / 國立陽明大學 / 解剖暨細胞生物學研究所 / 95 / Ischemia can affect the structure and function of organs and tissues in mammals. Brain ischemia may result from the impair of the cerebral blood flow and lead to hypoxia and hypoglycemia, which cause pathological damage of the brain. Hippocampus is vulnerable to ischemic lesions in experimental animals and human. The aim of this study is to investigate the morphological changes of blood vessels of the hippocampus by scanning electron microscopy and immunocytochemistry. After ischemic lesions, Mongolian gerbils, Meriones unguiculatus, were followed by bilateral common carotid artery occlusion and reperfusion at different time courses. Under the scanning electron microscopy observation, at the onset of reperfusion after 60 minutes ischemia, the collapse of lumen of the blood vessel and the perivascular space of the blood vessels became significantly dilated. At 6 hour reperfusion after 60 minutes ischemia, the perivascular space was reduced slightly and blood cells were congested in the collapsed vessel. A leakage route was found in the lining of continuous layer of endothelial cells. GFAP-like and S-100 like immunoreactivities (IR) were found mainly in the perimeter of the blood vessel in the stratum of lacunosum and moleculares of the sham-operated animals. In different time point, GFAP-like and S-100 like IR were decrease or increase. Our findings suggest that GFAP and S-100 may be involved in the regulation of morphological changes of blood vessels in the stratum lacunosum-moleculares after ischemic lesions.
| Identifer | oai:union.ndltd.org:TW/095YM005391005 |
| Date | January 2007 |
| Creators | Yu-Lin Chen, 陳羽琳 |
| Contributors | Shang-Ming Yu, 游祥明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
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