碩士 / 國立中正大學 / 化學所 / 96 / Gene therapy is an experimental technique that uses genes to treat or prevent disease. This technique may allow doctors to treat a disorder by inserting a gene into a patient’s cells instead of using drugs or surgery.
The goal of this project is to design and synthesize a biodegradable cationic poly( urethane-co-ethylenimine ) with low cytotoxicity and high transfection efficiency. The polycation / DNA complex not only can protect the DNA from nuclease degradation, but also has a nanoscale size small enough to enter the cell via endocytosis. The results revealed that PU-l PEI, PU-Gly-l PEI, PU-hPEI and PU-Gly-hpei could bind with plasmid DNA and yield positively charged complexes with a size required for transfection. The PU-hPEI, PU-Gly-hPEI /DNA complexes were able to transfect COS-7 cells in vitro with an efficiency comparable to a well-known gene carrier PEI. In this article, PU-hPEI, PU-Gly-hPEI seemed to be novel cationic polyurethanes for gene delivery and an interesting candidate for further study.
| Identifer | oai:union.ndltd.org:TW/096CCU05065025 |
| Date | January 2008 |
| Creators | Chung-mou Huang, 黃鐘謀 |
| Contributors | none, 程中玉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 121 |
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