碩士 / 嘉南藥理科技大學 / 化妝品科技研究所 / 96 / Marine soft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes, including anti-inflammatory, anti-microbial, anti-HIV activities and various cancers. However, the mechanism underlying its anti-oral cancer effect is poorly understood. This study evaluates the antiproliferative effects of ethyl acetate extracts of soft corals of the genus Sinularia (S. grandilobata, S. parva, S. triangular, S. leptoclados, S. scabra, S. depressan, S. nanolobata, S. inflata and S. gibberosa) towards human head and neck squamous cell carcinoma cells (SCC25) and premalignant keratinocytes (HaCaT). The cell adhesion assay indicates that nine extracts reduce the number of cell attachments with rising treatment concentrations (0-100 ?慊/ml). The S. parva extract is revealed to be the most effective inhibitor of cell adhesion. The genus Sinularia extracts exhibit dose-dependent cytotoxicity effect using MTS assay. The IC50 and IC80 values demonstrate that the S. parva and S. nanolobata extracts are the best inhibitors of cell proliferation. Treatment of extracts (IC50) to observe the morphological alterations in cells, membrane blebbing, the typical nuclear condensation, nuclear fragmentation and apoptotic bodies of cells are demonstrated with a phase contrast inverted microscope and Hoechst staining. Flow cytometry indicates that nine extracts sensitized SCC25 cells in the G0/G1 and S-G2/M phases with a concomitant dose-dependently increased sub-G1 peak. However, cell cycle analysis reveals that distinctly different from HaCaT cells, cells were locked in an S phase by S. leptoclados extracts, and the S-G2/M phase were arrested by S. depressan and S. inflata extracts. This apoptosis process was accompanied by up-regulation of p53 and activation of caspase-3 expression after SCC25 and HaCaT cells were treated with S. leptoclados, S. depressan and S. inflata extracts. Extracts of the genus Sinularia thus apparently cause apoptosis of SCC25 and HaCaT cells, and could be utilized as an anti-oral cancer cells activity drug for future studies.
| Identifer | oai:union.ndltd.org:TW/096CNUP5793017 |
| Date | January 2008 |
| Creators | Shih-Hao Wang, 王士豪 |
| Contributors | Chia-Hua Liang, 梁家華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 103 |
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