博士 / 中山醫學大學 / 生化暨生物科技研究所 / 96 / Functional foods can be defined as the foods contains special integrants which can improve the health condition and modulate biological function in human. The special benefit integrants in functional food verifies as the cultivated environment, process of extraction and restore. The goal of this research is to develop a dependable method to separate and determine the bioactive integrants in functional food.
During recent years, steroidal saponins have attracted a growing interest owing to the wide range of their biological action on living organisms,including hemolytic,hypocholesterolemic,hypoglycemic, anti-thrombotic, anti-antineoplastic, antiviral and anti-canceractivities.
Furostanol and spirostanol glycosides, two main steroidal saponins, have been found in many types of yams (Dioscorea species). Many reports indicated that steroidal saponins is one of the main bioactive compounds in yams (Dioscorea species). In this investigation, a spirostanol glycoside, (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl -(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-Dglucopyranoside,was isolated from wild yam (Dioscorea villosa) extract with reversed phase preparative HPLC. This was the first time found in yams (Dioscorea species). The structure was identified by LC-MS, 1H- and 13C-NMR. Cytostatic activities of the isolated saponin on Hep G2, HEK293 and MCF7 cells were estimated. Growth of these cells was inhibited as higher concentration of the compound was used. The IC50 values of the isolated spirostanol glycoside and its aglycon, diosgenin, were 9.02 and 23.91 μM for Hep G2 cells, 13.21 and 27.31 μM for HEK293 cells, and 16.74 and 35.38 μM for MCF7 cells, respectively. The isolated compound had significantly higher active intensity than diosgenin.
Dunaliella salina is a type of unicellular and halophilic green biflagellate microalga contains abundant β-carotene, For the determination of carotenoids, the reversed-phase high performance liquid chromatography (RP-HPLC) has been used.A simple HPLC method with good separation efficiency was developed to determine all-trans and cis forms of carotenoids in Dunaliella salina cultivated in Taiwan. The analysis used a C30 column (250×4.6 mm, 5 μm) and an isocratic solvent system (flow rate = 1 mL/min) mixing methanol–acetonitrile–water (84/14/2, v/v/v) and methylene chloride, (75/25, v/v). Carotenoids were detected at 450 nm. Moreover, the antioxidant capacities of the algal carotenoid extract were also evaluated with Trolox equivalent antioxidant capacity (TEAC) assay, reducing power and 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radical scavenging assay. Results showed that 7 carotenoids in the algal extract could be separated simultaneously within 30 min and the total amount of them was 290.77 mg/g algae. The contents of all-trans-β-carotene and 9- or 9’-cis-β-carotene, the major carotenoids in the algae, were 138.25 and 124.65 mg/g algae, respectively. The contents of all-trans-lutein, all-trans-zeaxanthin, 13- or 13’-cis-β-carotene, all-trans-α-carotene and 9- or 9’-cis-α-carotene were 6.55, 11.27, 4.95, 2.69, and 2.41 mg/g algae, respectively. The algal carotenoid extract had significantly higher antioxidant activity than all-trans forms of α-carotene, β-carotene, lutein and zeaxanthin in all antioxidant assays. The cis forms of carotenoids, especially 9- or 9’-cis-β-carotene, might play crucial roles for the antioxidant capacities of the algal extract.
| Identifer | oai:union.ndltd.org:TW/096CSMU5107023 |
| Date | January 2008 |
| Creators | Chao-Chin, 胡超群 |
| Contributors | Fen-Pi Chou, 周芬碧 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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