碩士 / 中山醫學大學 / 生物醫學科學學系碩士班 / 96 / Tiger prawn (Penaues monodon) is an important aqua-cultural species in Taiwan. It reaches its highest amount during the 1980s’. It suffered a serious loss resulted from high mortality rate of White spot syndrome virus (WSSV) infection during the 1990’s. Previous research indicated WSSV viral envelope protein, VP53A could interact with shrimp protein P. monodon chitin binding protein (PmCBP) through the yeast two hybrid method. CBP was first investigated in plant and the specialists found that CBP has both antifungal and antimicrobial activities. It could help the plant to resist the environmental stress and stay healthy. In invertebrate animals, they have the same protein which works to prevent the viral infection and bacterial attachment. In this research, PmCBP partial sequence was compared with other species and it has high similarity between them. And I try to understand the relationship between the antimicrobial PmCBP and the virus envelope protein VP53A through immune experiments. Western blotting was applied to detect the protein expression in shrimp tissues. In in vivo experiment, using WSSV infected shrimp hemocytes to react with immunofluorescence and observe the colocalization between PmCBP and VP53A. More detailed experiments are needed to discover the molecular mechanism.
| Identifer | oai:union.ndltd.org:TW/096CSMU5114006 |
| Date | January 2008 |
| Creators | Tai-Ching, 許台慶 |
| Contributors | 陳威仁 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 45 |
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