碩士 / 中山醫學大學 / 生物醫學科學學系碩士班 / 96 / Mycotoxins and phycotoxins are low-molecular-weight nonimmunogenic toxins, which are frequently found in our environment. They are potential hazards to human and animal health. This study focused on three different kinds of toxins, fumonisin B1 (FB1), aflatoxin M1 (AFM1) and microcystin-LR (MCY-LR), and developed an one efficient method to detect the toxin. We designed the method based on the specificity of antibody and antigen. For producing the polyclonal antibodies against each FB1, AFM1 and MCY-LR, each toxin was conjugated to the different carrier proteins and immunized the rabbits, respectively. A direct competitive enzyme-linked immunosorbent assay (cdELISA) was used for the characterization of the antibodies and for analysis of the toxins in the samples. The concentrations causing 50 % inhibition of binding of toxin-horseradish peroxidase (HRP) to the antibodies by FB1, AFM1 and MCY-LR in the cdELISA were found to be 0.71, 0.11 and 0.17 ng/mL, respectively. Because cdELISA have to be analysed in the laboratory, it is needed to develop an assay that could be widely used in on-site screening of samples. Therefore, our study is focused on the development of gold-nanoparticle based rapid immunochromatographic strip, which is simpler and less time-consuming than cdELISA. In this study, both gold-nanoparticle based rapid immunochromatographic strips suitable for on-site FB1 and AFM1 determination were established with the detection limit of 5 ng/mL. Results of samples analysis obtained from gold-nanoparticle based rapid immunochromatographic strips were in a good agreement with those obtained from cdELISA. The cdELISA and gold-nanoparticle based rapid immunochromatographic strip can be rapid and efficient to detect the toxins in the samples.
| Identifer | oai:union.ndltd.org:TW/096CSMU5114008 |
| Date | January 2008 |
| Creators | Jing-Jhih, 王敬之 |
| Contributors | Feng-Yih Yu, 余豐益 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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