碩士 / 國立中興大學 / 化學工程學系所 / 96 / The effects of nitrogen and carbon sources on the production of chlorophyll and phycocyanin by spirulina platensis were investigated under red light-emitting diode (LED) with 3000μmol photons m-2 s-1 as the light source.
From the experimental results, nitrogen deficiency resulted in phycocyanin and chlorophyll degradation, which provide amino acid for protein biosynthesis or other important cellular constituents for cell growth. Urea addition at different time interval for Spirulina platensis growth was examined. The result showed urea was advantageous for phycocyanin accumulation in algea.
The data also displayed that urea concentration higher than 0.25 g/L would hinder Spirulina platensis growth. At urea concentration of 0.175 g/L, and NaHCO3 concentration of 8 g/L, the phycocyanin content per biomass increased 28％ as compared to that in basic culture medium.
On the other hand, the sodium nitrate in basic culture medium was not consumed during culture period, which means it exerts no effect on the cell growth. When using 0.1 g/L of urea as the sole carbon and nitrogen source in the cultivation, the algae biomass reached 0.33 g/L.
When specific urea consumption rate was less than 0.05 g/g/day, both of specific chlorophyll and phycocyanin production rate values are negative, and the phycocyanin specific degradation rate is higher than that of chlorophyll. Besides, at the same specific urea consumption rate, the specific phycocyanin production rate is 7 times higher than that of chlorophyll.
| Identifer | oai:union.ndltd.org:TW/096NCHU5063043 |
| Date | January 2008 |
| Creators | Wan-Lin Weng, 翁婉琳 |
| Contributors | Yung-Chuan Liu, 劉永銓 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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