碩士 / 國立中興大學 / 食品暨應用生物科技學系 / 96 / The purpose of this study was to investigate the stability of fucoxanthin in different solvent systems and the cellular uptake in two types of liver cells. A ethanol was used first to prepare a 10 mM fucoxanthin solution, which was then further diluted to 1.0 mM in ethanol (Stock A) or in 100% FBS (Stock B). A 10 μL Stock A was added to 10 mL DMEM medium containing 10% FBS (A1) or without containing FBS (A2). Likewise, A 10 μL Stock B was added to 10 mL DMEM medium containing 10% FBS (B1) or without containing FBS (B2). The final concentration of fucoxanthin in these solvent systems was 10 μM.
We found that the fucoxanthin in the four different solvent systems degraded with increasing incubation time (3-72h). Fucoxanthin in group A2 had the best stability, with 73.3% of fucoxanthin remaining at 72 h, while fucoxanthin in group B1 had the poorest stability, with only 34.9% fucoxanthin remaining at 72 h.
Regarding cellular uptake in SK-Hep-1 cells, fucoxanthin in all aolvent systems had the highest uptake at 24 h, at which time fucoxanthin in group A2 had the highest uptake (9.81%), while group A1 had the lowest uptake (2.10%). For BNL CL.2 cells, group B1 had the highest uptake (5.6%), while group A1 had the lowest uptake (2.91%) at 24 h. In the HPLC profile, there was an unknown peak, which was suspect to be a metabolic product of fucoxanthin by cells. The concentration of this product reached the maximum level at 24 h and 48 h of incubation for SK-Hep-1 cells and BNL CL.2 cells, respectively.
In addition, we noted fucoxanthin-induced changes in cell morphology. With the increase in incubation time, SK-Hep-1 cells became deformed and had low viability, especially when incubated in the FBS-free medium (groups A2 and B2). Fucoxanthin also affected the morphology of BNL CL.2 cells by causing deformation and production of vesicles, even when FBS was present (groups A1 and B1).
In summary, this study shows that the stability was better when the fucoxanthin stock was prepared in ethanol and then added into the FBS-free medium (group A2), which also has the best cellular uptake of fucoxanthin in SK-Hep-1 cells. In BNL CL.2 cells, however, cellular uptake of fucoxanthin is better in B1 group, which was prepared in 100% FBS and then diluted in DMEM medium containing 100% FBS.
| Identifer | oai:union.ndltd.org:TW/096NCHU5255043 |
| Creators | Ai-Ling Liang, 梁愛羚 |
| Contributors | Miao-Lin Hu, 胡淼琳 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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