碩士 / 國立成功大學 / 化學系碩博士班 / 96 / Collagens (acid-solubilized collagen, ASC；pepsin-solubilized collagen, PSC) , gelatin and collagen hydolysate were prepared from hybrid tilapia skin and partially characterized. The yield of collagen was improved due to the addition of pepsin in the purification process. Only ASC and PSC possessed a triple helical conformation. Under low temperature condition, the digestion of collagen by pepsin improves its water solubility. On the other hand, collagens exhibited many properties distinct from gelatin and collagen hydrolysate, such as viscosity ,molecular weight distribution, basic isoelectric point, secondary structure, and ability of fibril formation. Gelatin and collagen hydrolysate were the denatured products of native collagen and adopted a random coil conformation, showing lower resistance to pepsin. Collagen isolated from Taiwan Hybrid Tilapia skin can be a high value product due to its special characteristics and has many potential future applications in biomaterials, functional additives, cosmetics and pharmaceutical industries.
| Identifer | oai:union.ndltd.org:TW/096NCKU5065109 |
| Date | January 2008 |
| Creators | Reng-Long Yang, 楊人龍 |
| Contributors | Shih-yu Saio, 蕭世裕 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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