碩士 / 國立彰化師範大學 / 生物學系 / 96 / Over the years, researchers have found there are different CHH (crustacean hyperglycemic hormone) molecules in crustaceans. According to those studies there were six CHH/CHH-like peptides in crayfish (Procambarus clarkii ) ：CHH1、CHH1-L、CHH2、CHH2-L、Hem-CHH1 and Hem-CHH2. Methods of molecular cloning resulted in full length sequence of hemocyte CHHs (involve untranslated region, UTR). Moreover with RT-PCR analysis, these CHH/CHH-like peptide genes were widely expressed in many tissues (hemocyte, eyestalk ganglia, cerebral ganglia, circum-oesophageal nerve, thoracic ganglia, abdominal ganglia, gut, heart and muscle). On the other hand, we investigated the effects of lipopolysaccharide (LPS) on total hemocyte count (THC) and CHH2-L gene relative expression. We gained the threshold cycle (Ct) of target gene and normalized with reference gene Ct, then compared target gene’s relative expression ratios of different samples. Following LPS injection (up to 24 hours post-injection) THC decreased significantly and CHH2-L transcript levels increased significantly over time after LPS injection. Afterward we detected three CHH (CHH2-L, Hem-CHH2 and CHH1-L) relative expression in different nervous tissues after LPS injection, found CHH2-L and Hem-CHH2 transcripts levels were higher than those in none-injection samples. The combined results suggest that CHH structural variants widely expressed and at least for CHHs might be involved in immunomodulation.
| Identifer | oai:union.ndltd.org:TW/096NCUE5112032 |
| Date | January 2008 |
| Creators | Jia-Ying Jiang, 江佳穎 |
| Contributors | Chi-Ying Lee, 李奇英 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 114 |
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