碩士 / 國立彰化師範大學 / 生物學系 / 96 / Cadmium (Cd) not only affects human health, but also is toxic to aquatic organisms, inhibiting their growth and changing their physiology and metabolism. Many studies showed that Cd-induced toxicity in plant is linked to lipid peroxidation and increases in malondialdehyde (MDA) content. The green algae Chlorella sp. is easy to culture with a short reproductive cycle, and therefore is a good experimental material. The objective of this study was to examine the effects of Cd on lipid peroxidation and the antioxidation ability of polyamines in synchronous cultures of Chlorella sp. For initial synchronous cultures of Chlorella sp. with cell densities of 1.56 × 106, half maximal effective concentration (EC50) of Cd was approximately 386 μM.
Treatment of synchronous cultures of Chlorella sp. with cell densities of 2.4 × 107 at the 12th hour light cycle with 200 and 400 μM of Cd for four hours resulted in increases in proline content, decreases in protein content, but polyamines (Put and Spd) and MDA contents were not changed. Treatment of synchronous cultures of Chlorella sp. with cell densities of 2.4 × 107 at the 12th hour light cycle with 1200 μM of Cd for two hours resulted in increases in proline content, increases in MDA content, increases in SOD activity, and decreases in APOX, CAT and GR activity, but polyamines (Put and Spd) contents were not changed.
Treated with Put, Spd and inhibitors of polyamines for 1 hour after the 11th hour light cycle, and exposed to 1200 μM of Cd for two hours resulted in increases MDA content. But 1 mM proline treatment resulted in decreases MDA content significantly. Treated with D-Arg resulted in increases MDA content significantly, but not MO. Treated with D-Arg + Put or D-Arg + Spd resulted in decreases MDA content when compared with treatment Put or Spd. Treated with Spd and proline for 1 hour after the 11th hour light cycle, and exposed to 1200 μM of Cd for two hours resulted in recover CAT and GR activity, and proline treatment resulted in increases SOD significantly. Treated with Spd for 1 hour after the 11th hour light cycle, and exposed to 1200 μM of Cd for two hours resulted in increases proline content, and proline treatment resulted in increases Put, but not Spd. However, treatment of proline could improve lipid oxidative of Cd stress, and polyamines may play a indirect role.
| Identifer | oai:union.ndltd.org:TW/096NCUE5112038 |
| Date | January 2008 |
| Creators | Chun-Lang Chen, 陳俊郎 |
| Contributors | John-Yee Lin, Wei-Long Wang, 林忠毅, 王瑋龍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 102 |
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