碩士 / 國立東華大學 / 海洋生物科技研究所 / 96 / In continuing search the bioactive secondary metabolites from marine microorganism, we found that the extracts of marine cyanobacteria Lyngbya majuscula, collected from Ryukyu Island, showed significant anticancer and anti-dialetes activities. Through bioassay-guided separation, we isolated four malyngamides, including three new compounds, malyngamide Z (1), malyngamide X (2), malyngamide 1 (4) and a known compound, isomalyngamide A (3). We also isolated four aplysiatoxins, including one new compound, 3-methoxy debromoaplysiatoxin (7) and three known compounds, ahhydrodebromoaplysiatoxin (6), debromoaplysiatoxin and oscillatoxin B (8) from L. majuscula. The structure of these compounds were determined by 1D and 2D NMR spectroscopic analysis.
In the biological test, compound 1、3 and 5-8 exhibited activity in cell lines of human hepatoma 2 (Hep2) and human colorectal cancer (WiDr). The ED50 values were between 11.6 ~ 26.0µg/ml. In brine shrimp toxicity test, malyngamides (1、3、4) showed no activity and aplysiatoxins 5 ~ 8 showed strong activity. The LD50 values were between 0.5 ~ 7.7 µg/ml.
| Identifer | oai:union.ndltd.org:TW/096NDHU5270005 |
| Date | January 2008 |
| Creators | Jine-Yung Huang, 黃俊揚 |
| Contributors | Chung-Kuang Lu, 盧重光 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 106 |
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