碩士 / 國立宜蘭大學 / 生物技術研究所碩士班 / 96 / By definition, viruses are unable to replicate within a host cell. Virus infection
can trigger events that lead to apoptosis, however some viruses are known to encode
gene products or induce host gene products that block apoptosis and use the host-cell
macro-molecular machinery and energy supplies to replicate. In previous studies, it
was suggested that GIV could induced apoptosis in barramundi muscle cells via
caspase-3, -8, -9 activation pathway.
In this study, a proteomic method was applied to identify alterations in protein
expression profile in barramundi muscle cells and being an indicator of apoptosis
after GIV infection. From this screening, 7 novel proteins were identfied, including
Initiation factor 4A 1B （eIF4A1B）, Proteasome 20S, ADP-ribosylation factor 1
（Arf-1）, Ribose 5 phosphate isomerase（RPIA）, Tyrosine Hydroxylase（TH）, RAN
binding protein 1, and Phosphoglycerate mutase 1（PGAM1）, were overexpressed in
GIV-infected barramundi muscle cells. In addition, we also determined these genes
expression levels by real-time PCR. The results showed that except Initiation factor
4A 1B （eIF4A1B）but, ADP-ribosylation factor 1（Arf-1）, Ribose 5 phosphate
isomerase （RPIA）and Tyrosine Hydroxylase （TH）were up regulated conpared to
the mock-infected cells. However, the Phosphoglycerate mutase 1（PGAM1）
expression only rises slightly is heightened in comparison to the mock-infected cells.
Summarized our results indicated that GIV could induce host genes expression
level, by GIV infection and supports viral, and replication virus-infected cells of
premature death. Our results provide an information for understanding of GIV viral
replcation in the host cells and possible mechenisms apoptosis regulatory systems.
| Identifer | oai:union.ndltd.org:TW/096NIU07108004 |
| Date | January 2008 |
| Creators | Chen,Chin-Wen, 陳瑾玟 |
| Contributors | Yu-Shen Lai, 賴裕順 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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