碩士 / 國立宜蘭大學 / 食品科學系碩士班 / 96 / Organisms can produce antimicrobial materials as the first line of defence to resist microorganism invading and infection. A group of proteins, which are capable of resisting microorganism infection, named antimicrobial peptide (AMP), have been found in plants, invertebrates and vertebrates. The grouper epinecidin-1 protein with 67 amino acid residues can inhibit Gram-positive and Gram-negative bacteria. Plasmid DNA containing cytomegalovirus (CMV)-promoter-driven EGFP and epinecidin-1 was administered into muscle of Zebrafish (Danio rerio) by varying electroporation containing number of pulses, voltage and plasmid concentration. To demonstrate the expression of EGFP-epinecidin-1 in the muscle at the site of injection, RT-PCR and immunohistochemistry staining techniques were applied. Foreign DNA was detected by polymerase chain reaction in the injected muscles and other tissues. In addition, we also found that EGFP-epinecidin-1 gene was efficiently expressed in vivo against Vibrio vulnificus 204 significantly (p < 0.01). Signal and cytokine regulation demonstrated that CMV-gfp-epi gene transfer could reduce LPS-induced proinflammatory responses in vivo. This technology may substitute the traditionally used chemotherapy agents and antibiotics in the future and reduce risk to human health.
| Identifer | oai:union.ndltd.org:TW/096NIU07253016 |
| Date | January 2008 |
| Creators | Tzu-Wen Fan, 樊姿玟 |
| Contributors | Shih-Bin Lin, Jyh-Yih Chen, 林世斌, 陳志毅 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
Page generated in 0.0167 seconds