碩士 / 國立高雄海洋科技大學 / 海洋環境工程研究所 / 96 / Most of the current methods of preprocessing sulfonamides in various substrates are based on liquid-liquid extraction, solid-phase extraction, and supercritical fluid extraction. These preprocessing procedures may require a large amount of organic solvents, long processing time, and complicated operations. The extant studies of sulfonamides have focused more on sulfonamides in chicken, swine, beef, viscera, by-products or forage but less on fish.
Therefore, this study applied microwave-assisted extraction (MAE) to extract eight kinds of sulfonamides commonly used in the aquaculture industry, including sulfadiazine (SDZ), sulfamethoxazole (SMX), sulfamerazine (SMR), sulfamethazine (SMT), sulfamonomethoxine (SMM), sulfathiazole (STZ), sulfadimethoxine (SDM), and sulfaquinoxaline (SQX) and to obtain the optimal conditions for applying on fish. In our experiment, high performance liquid chromatography (HPLC) was used to perform the analysis. The sample was prepared by 0.2 g blank fish meat added with 10 μg/g sulfonamides for 4 hours, the optimal condition can be derived according to the single variable method. Extracting sulfonamides with 10 mL acetonitrile under 160 ℃/6min. The recovery ratio is between 82.6 % ~ 111.6 % with a relative standard deviation below 4%. The detection limitation is between 0.0319 μg/g and 0.0901 μg/g which are lower than the tolerance of veterinary sulfonamides 0.1 μg/g. So the proposed method is suitable for sulfonamides evaluation.
In this study, a closed MAE method was proposed and applied to extract eight kinds of sulfonamides in fish. Compared with the CNS14459 method, the proposed method not only accurate (recovery rate between 82.6 % ~ 111.6 %) but also précised (relative standard deviation below 4 %) and simple preprocessing operations (adding the sample into the extracting solvent and put the solution in the microwave extraction equipment), are required small amount of solvent (10 mL acetonitrile), and short extraction time (only 6 minutes). In addition to the burden on the operation staffs, extraction time, solvent consumption, and secondary pollution can also be reduced.
| Identifer | oai:union.ndltd.org:TW/096NKIM8282002 |
| Date | January 2008 |
| Creators | Chou Wei-Zhi, 周威志 |
| Contributors | Chen Chiu-Yun, 陳秋雲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 63 |
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