碩士 / 國立屏東科技大學 / 水產養殖系所 / 96 / Cortisol is mainly produced form 11-deoxycortisol by 11β-hydroxylase in adrenal cortex of higher vertebrates or in the inter-renal tissue of fish, and is secreted into blood. Cortisol is associated with many physiological functions including energy metabolism, ion regulation and stress response. Previous physiological studies have proposed that cortisol may regulate calcium uptake in fish through epithelia calcium channel (ECaC), but the detailed mechanism is still unclear. Zebrafish is an excellent animal model in biological research because it has plenty of genetic database and is applicable in various molecular physiological approaches. In this study, we used zebrafish as the model to further explore the mechanisms how cortisol regulates calcium uptake in fish. Exogenous cortisol was found to increase body length and calcium content, and also to upregulate the mRNA expressions of ECaC and 11β-Hydroxysteroid dehydrogenase type 2 (11β-HSD2) in zebrafish embryos. However, the treatment of cortisol was shown to downregulate the mRNA expressions of cortisol receptors (mineralocorticoid and glucocorticoid receptor) and 11β-hydroxylase. Taken all together, cortisol may regulate fish calcium uptake via modulating upstream and downstream steroidgenic enzymes, receptors and target ion transporter.
| Identifer | oai:union.ndltd.org:TW/096NPUS5086002 |
| Date | January 2008 |
| Creators | Tsai I-Lun, 蔡依倫 |
| Contributors | Pung-Pung Hwang, Mei-Chen Tseng, 黃鵬鵬, 曾美珍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 37 |
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