碩士 / 國立屏東科技大學 / 獸醫學系所 / 96 / Koi herpesvirus ( KHV ) is an important viral disease in koi ( Cyprinus carpio koi ) and common carp ( Cyprinus carpio carpio ). From 2003 to 2005, 40 cases of common carp and 9 cases of koi carp farms were collected in Tainan. Those cases were diagnosed by PCR examination, 37 cases were identified as KHV infection, total positive rate of KHV infection in carp is 75.5% [ carp cases 75% ( 30/40 ) and koi cases 77.8% ( 7/9 ), respectively ]. Through investigating, the clinical signs include lethargic, anorexic, increased respiratory movements and moving slowly in diseased fish. The incubation period of KHV infection is about 2 weeks, and the cumulative mortality of fish infected with koi herpesvirus ( KHV ) were 70~100%, the total cumulative mortality was 82.3% ( carp farms 80.3% and koi farms 90.7% ). The gross finding were hyperemia and haemorrhage on the body, pale and necrosis in gill filaments. The histopathological lesions of total KHV cases of this study include: epithelial hyperplasia, fusion ( 86.5% ) and necrosis ( 35.1% ) in gill filaments, eosinophilic intranuclear inclusion body were also found; focal necrosis lesion in liver ( 10.8% ); focal necrosis lesion in spleen
( 37.8% ); haemorrhage in kidney ( 13.5% ); degeneration ( 18.9% ) and necrosis ( 13.5% ) in renal tubular cells; Zenker’s necrosis in heart muscles ( 8.1% ). Amplify specific DNA fragment of 37 samples by PCR ( KHV TK： 410 bp、KHV 9/5：483 bp ), alignment report present their nucleic acid sequences were identical, koi herpesvirus infection in koi and common carp in Tainan might be the same origins. In KHV 9/5 gene sequences, they exhibited identities of 100% with Japanese strain ( accession number AP008984 ), 99% with Israeli strain ( accession number DQ177346 ) and 99% with USA strain ( accession number DQ657948 ). The koi herpesvirus infection in Tainan were most closely relationship with Japanese strain. This research present koi herpesvirus infection month from November to April next year ( weather temperature 17.5~25.3℃, average temperature 20.9℃ ), and it is only seldom cases in May to October with higher temperature ( average temperature 28.3℃ ). Therefore, these results suggested that the LAMP assay might be more sensitive than PCR test. LAMP is a rapid diagnostic method for primary examined KHV in clinic.
| Identifer | oai:union.ndltd.org:TW/096NPUS5541002 |
| Date | January 2007 |
| Creators | Chung-Yi Chen, 陳峻鎰 |
| Contributors | Shih-Chu Chen, 陳石柱 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 96 |
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