碩士 / 國立臺灣師範大學 / 生命科學研究所 / 96 / Planktonic organisms can be subdivided based on dimensional criteria, and picoplankton are defined on 0.2-5 μm size-scale organisms. Picoplankton are distributed worldwide and are ubiquitous in all types of waters, including autotrophic picoplankton and heterotrophic picoplankton. Autotrophic picoplankton are major players in carbon production in all aquatic ecosystems. Recently picoplankton are popular subjects in research, and most of them are interested in nutritive values, physiological characterizations, ecological roles, and categorization. In those studies, most are aimed at known picoplankton, but there are lots of unknown species in biosphere.
In this study, picoplankton were isolated in water samples, and furthermore be categorized by morphology and physiological characterizations. There are three parts in this study: (I) Morphological observations in microscopy and scanner electric microscopy. (II) Categorization by blasting of 16S sequence. (III) Physiological characterizations by determining growth rates and the level of photosynthetic pigments of isolated picoplankton in different light conditions.
According to the data of this study, picoplankton from different depth of the water in Fei Cui reservoir are also different in morphology and physiological characterizations, suppose the pressure of the depth of the water affect. In addition,
the conclusion also advice that the best length of 16S sequence is more than 1500 bp for more effective blasting.
| Identifer | oai:union.ndltd.org:TW/096NTNU5105052 |
| Date | January 2008 |
| Creators | 沈家玉 |
| Contributors | Yung-Ta Chang, 張永達 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 55 |
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