碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / This study investigated the efficacies of complex vaccines derived from inactivated Photobacterium damselae subsp. piscicida (Ph.d.p.) and/or Vibrio alginolyticus (V. al), originally isolated from disesed cobia , in cobia after various immunizations. The bacteria were cultured on various media including YFP, YEP supplemented with or without EDDHA.
Groups of cobia immunized with YEP and YFP (without EDDHA ) prepared complex vaccines via intraperitoneal (i.p.) injection exhibited good protective efficacies after challenge with Ph.d.p. or V. al at week 8. The fish immunized with YFP (without EDDHA) prepared vaccines (i.p. injection) exhibited good protective efficaaies after challenge with Ph.d.p. or V. al at week 12. The fish immunized with YFP (without EDDHA) prepared vaccine (i.p. injection) exhibited good protective efficaaies after challenge with V. al at week 12. No good protective efficacy could be obtained in fish immunized with vaccines preparing from bacteria grown on YEP or YFP supplemented with EDDHA after challenge with both bacteria. Meanwhile, no good protective efficacy could be obtained in fish immunized with vaccines (via immersion) preparing from bacteria grow on YEP or YFP supplemented with or without EDDHA after challenge with both bacteria. The results revealed that cobia immunized with the complex vaccine of YFP and YEP (without EDDHA) via intraperitoneal (i.p.) injection exhibited good protective efficacies after challenge with Ph.d.p. or V. al, and are good candidates for further development of commercial vaccines.
| Identifer | oai:union.ndltd.org:TW/096NTOU5086002 |
| Date | January 2008 |
| Creators | Jia-Rung Chang, 張家榮 |
| Contributors | Ping-Chung Liu, 劉秉忠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 95 |
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