碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Abstract
The experiment was conducted to investigate the effects of dietary supplementation with different carotenoids on skin pigmentation, growth and body carotenoids content of tomato anemonefish, Amphiprion frenatus. We focus on three axes in this study. First, we studied the effect of diets with different astaxanthin level (0、200、400、600、800、1000 or 1200 mg/kg) on 4 months tomato anemonefish (1.36±0.03 g) during 65 days. In the second experiment, diets with constant astaxanthin concentration (400 mg/kg) and different zeaxanthin level (0、200、400、600、800、1000 or 1200 mg/kg) were tested on 4 months tomato anemonefish (1.66±0.01 g initial weight) during 60 days. Finally, we separate the wild species of tomato anemonefish into two groups, Black and Red. For both groups, color parameters were compared with the fishes used in experiment one and experiment two.
The first experiment revealed an increase of the skin pigmentation parameter CIE (1976) a* with increasing astaxanthin concentration for the first 45 days. Over 45 days, this parameter reached its maximum value for astaxanthin concentration of 400 mg/kg and above. At the 15 days of experiment, the astaxanthin concentration reached its maximum value in fish body for all treatments. However, each treatment were significantly. This experiment also showed that fish body total carotenoids concentration continuously increased with astaxanthin concentration and over the 60 days of experiment. At the end of the experiment, the final weight, weight gain and FCR didn’t show any difference between each treatment, only SGR did.
For the second experiment, we observed an augmentation of skin pigmentation parameter CIE (1976) b* with zeaxanthin concentration for 30 days. Over this period, this skin parameter CIE (1976) b* exhibited, as CIE (1976) a* with astaxanthin in the first experiment, a pattern of saturation. But here, the maximum value was reached for zeaxanthin concentration over 200 mg/kg. No effect of zeaxanthin were observed on CIE (1976) a*, this later followed similar trend as in the first experiment for 400 mg/kg of astaxanthin concentration. In this experiment, both astaxanthin and zeaxanthin reached their maximum value in fish body for all treatments at 15 days. Nevertheless, each treatment could not be statistically differentiated. Moreover, this experiment showed that zeaxanthin had analogous effect as astaxanthin on fish body total carotenoids concentration. Concerning the final weight, weight gain, FCR and SGR, none of them displayed significant difference.
Combining the results of the two first experiments, additional results were obtained. First, we shown that there is a positive logarithmic relation between skin parameters CIE (1976) a*, b* and C* and body total carotenoids concentration. Moreover, an increase of carotenoids concentration in the diet reduced the body carotenoids retention percentage.
From the last experiment, we concluded that the colour parameters were higher in the fish raised in experiment one and two, as well as the wild red group compare to the wild black group. On the whole, we showed that only the use of the food in experiment two allowed to obtain similar color to the wild red anemone fish.
| Identifer | oai:union.ndltd.org:TW/096NTOU5086018 |
| Date | January 2008 |
| Creators | Jian-Tong Chen, 陳建同 |
| Contributors | Fan-Hua Nan, 冉繁華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 138 |
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