碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Grouper is high economic species of aquaculture in Taiwan, and disease prevention is vital issue to grouper culturing as infectious nervous necrosis virus has caused great damages to aquaculture. Innate immunity is early development than acquired immunity which character of immediately, non-specific and temperature-independent. Mx and AMPs (antimicrobial peptides) belongs to innate immunity and its antivirus and antibacterial activity has been confirmed in some species. The aims of present study is to elucidate antiviral activity of AMP and Mx and the impacts of environment factor and various immunostimulants on AMP and Mx gene expression in groupers (Epinephelus spp.). In the present study, giant grouper AMPs has shown the inhibitory and bactericidal activity of bacteria, fungus and nervous necrosis virus by susceptibility test and neutralization test. The expression level of Mx and AMP genes were induced by reducing salinity and intraperitoneal injection of shikimic acid in adult grouper. The mRNA level of Mx and AMP genes was significant lower in the larvae which smaller than 2 inch in length. The Mx and AMP mRNA level can be induced by peptidoglycan and β-1,3-glucan in grouper larvae. Furthermore, Mx and AMP gene expression arised after feeding with Chinese herbs medicine such as Isatis indigotica, Astragalus membranaceus, Glycyrrhiza uralensis, and complex Chinese herbs (yin qiao san). Employment of poly I:C and Chinese herbal medicine to induce Mx and AMP gene can reduce the mortality on NNV and Vibrio alginolyticus infection grouper larvae.
| Identifer | oai:union.ndltd.org:TW/096NTOU5086035 |
| Date | January 2008 |
| Creators | Guan-Min Chen, 陳冠民 |
| Contributors | Jenn-Kan Lu, 陸振岡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 115 |
Page generated in 0.0015 seconds