碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 / The inhibitory effects of Chlorella hydrolysates (hydrolyzed by cellulase and protease) against α-glucosidase were investigated and the physiological effect on regulation of blood sugar was also evaluated. The carbohydrate, soluble protein and peptide content of three Chlorella hydrolysates (pulverized cell, unpulverized cell and residue) were increased as cellulase was added to 5%. The hydrolysate (pulverized cell) showed the highest yield being 21.40%, and α-glucosidase IC50 was 27.48 mg/mL. The hydrolysate (pulverized cell) was separated into three fractions by size exclusion chromatography on a Sephadex-50 column. The third fraction of the hydrolysate showed the lowest α-glucosidase IC50 being 0.103 mg carbohydrate/mL and 0.038 mg peptide/mL (the drug of Acarbose was 0.394 mg/mL). The yield of hydrolysates increased to 41.02 (pulverized cell), 45.78 (unpulverized cell) and 32.01% (residue), respectively, as by 5% Cellulase AP3 and 2% Protease N hydrolyzed. Then, the hydrolysate (pulverized cell) was separated into three fractions by size exclusion chromatography on a Sephadex-15 column. The third fraction of the hydrolysate showed the lowest α-glucosidase IC50 being 0.074 mg carbohydrate/mL and 0.099 mg peptide/mL. The third fraction was further analyzed by HPLC, only peak Ⅲ-1 had inhibitory effect on α-glucosidase and inhibitory efficiency ratio (IER) was 2531.8 %/mg/mL.
| Identifer | oai:union.ndltd.org:TW/096NTOU5253019 |
| Date | January 2008 |
| Creators | Chia-Hui Wang, 王佳慧 |
| Contributors | Jenn-Shou Tsai, 蔡震壽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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