碩士 / 國立臺灣大學 / 海洋研究所 / 96 / Saturated hydrocarbons, including alkanes and cycloalkanes, constitute the largest fraction of crude oil by mass. In this study, the water samples were cultured in CM broth within hexadecane or cyclohexane. There were 32 isolates of bacteria which could degrade hexadecane or cyclohexane isolated from northeast coast, Shiangshan and South Bay. It could be classified into thirteen genotypic groups by the RFLP analysis. According to the 16S rRNA gene sequencing results, these isolates belong to Marinobacter, Vibrio, Shewanella, Pseudoalteromonas, Halomonas, Pseudomonas and Prolixibacter. Phenotypic and phylogenetic data of the isolates reveal that one isolate, HD54, could be novel genus.
Strain HD54 was non-motile, non-flagellated, rod-shaped, pink-orange bacterium. Cells were gram negative, aerobic, catalase- and oxidase-positive. On the basis of 16S rRNA gene sequence phylogeny Prolixibacter bellariivorans is only 89.4 % similar to HD54. Results from physiological, biochemical and phylogenetic analysis showed that HD54 should be classified as a novel genus and species within the phylum Bacteroidetes.
This study showed high diversity of bacteria degrading hexadecane or cyclohexane in coastal waters of Taiwan. It might have the potential of applicability. It is worth to study further in the future.
| Identifer | oai:union.ndltd.org:TW/096NTU05274017 |
| Date | January 2008 |
| Creators | Min-Hsiao Wu, 吳旻曉 |
| Contributors | 謝文陽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 42 |
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