博士 / 國立臺灣大學 / 動物學研究所 / 96 / The black tiger shrimp (Penaeus monodon) is an ecologically and economically important marine crustacean and is widely distributed in the Indo-Pacific region. Here I investigated the genome of P. monodon in two aspects, i.e., (1) the genetic diversity of wild populations, (2) the construction of a genetic linkage map.
There are 4 chapters in this thesis. Chapter 1 contained the paper review of the research topics, the research methods, and the research aspects. In chapter 2 the genetic diversity and the stock identification of wild P. monodon populations in Indo-Pacific region was revealed, and this part of work has been published in Animal Genetics. In chapter 3 the constructed linkage maps of the tiger shrimp based on microsatellites and AFLP markers were presented. Chapter 4, the final chapter, was concerned with the research summary and future perspectives.
In order to investigate the genetic diversity of wild P. monodon populations, tiger shrimps (n = 355) from eight geographical regions were genotyped using of 10 microsatellite markers. A high level of genetic variability (average HO: 0.638-0.743) and significant genetic differentiation (Pairwise FST and RST values) among the populations were revealed. The non-metric multidimensional scaling analysis indicated the presence of three geographic groups in the Indo-Pacific region, i.e., the African populations, a population from western Thailand, and the remaining populations as a whole. In addition, the phylogenetic analysis using the sequences from the mitochondrial control region (mtCR) revealed that the West Indian Ocean populations were genetically differentiated from the West Pacific populations, consistent with the result obtained from the microsatellite data.
In order to acquire more genetic information of tiger shrimp genome, the linkage maps of male and female tiger shrimp (P. monodon) were constructed based on microsatellite and amplified fragment length polymorphism (AFLP) markers. 621 microsatellite and 1151 AFLP markers were tested for polymorphism. Consequently, 372 (167 microsatellite and 203 AFLP markers) were used for genotyping an isofemale F1 family comprising two parents and 280 progeny. From the linkage analysis (LOD score= 4.5), a male and a female linkage map consisting of 43 and 46 linkage groups were constructed, respectively. The male map consisted of 126 microsatellite markers and 79 AFLP markers, whereas the female map consisted of 128 microsatellite markers and 55 AFLP markers. These two maps shared 36 homologous linkage groups.
| Identifer | oai:union.ndltd.org:TW/096NTU05312014 |
| Date | January 2008 |
| Creators | En-Min You, 尤恩民 |
| Contributors | Hon-Tsen Yu, 于宏燦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 62 |
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