碩士 / 國立臺灣大學 / 動物學研究所 / 96 / Brevetoxins (PbTx) are the major neurotoxin produced by algae when red tide happens and are classified according to their chemical structure named Brevetoxin-A(PbTx-A) and Brevetoxin-B(PbTx-B). Researches in the past believe that these toxins bind to Na+ channel and affect its activation and inactivation. Reports in the past focused on the classification, purification and detection of the toxin. For the electrophysiology study, crafish and squid giant axon were used as model systems. Yet few people used neuroendocrine cells as the model system to study the effect of the toxin to Na+ channel modulation, exocytose secretion and firing of action potential. In this report we use bovine adrenal chromaffin cells to test whether its electrophysiological feature will change after toxin treatment. Our results showed that 1 uM of PbTx-1, one kind of PbTx-A, shifted the inactivaion and decreased the conductance of Na+ channel in bovine chromaffin cells. These results indicate that PbTx may modulate the action potential firing, exocytosis and endocytosis in neuroendocrine cells.
| Identifer | oai:union.ndltd.org:TW/096NTU05312019 |
| Date | January 2008 |
| Creators | Chih-cheng Yang, 楊智誠 |
| Contributors | Chien-Yuan Pan, 潘建源 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 32 |
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