碩士 / 國立臺灣大學 / 動物學研究所 / 96 / Taiwan is located at the northern edge of the Coral Triangle where marine biodiversity is the richest in the world. There are about 3000 species of fish recorded in Taiwan, which is one tenth of the world’s total species. The supply of fishery resources is related to the recruitment of fish eggs and larvae. Thus the studies of fish early life history and egg identification are important for the resource conservation and management. In this study, a series of developmental morphology of marine fish eggs were observed using the hatching method and verified with molecular identification utilizing DNA barcoding to assess the feasibility and efficiency of the hatching method. The data collected also contributed to a better understanding of species composition, temporal and spatial distribution of fish eggs in the waters off Taiwan. From total of 104 hatched fish eggs, the successful rate of the hatching method is 70 %. These 104 fresh fish eggs were classified into 38 types, including 5 to orders, 14 to families, 5 to species, and 14 unknown types, a total of 24 taxa by their morphological characteristics. Eggs were identified into 41 types, including 27 taxa after hatched to preflexion larvae. However, when applying DNA barcoding, fish eggs or larvae were classified into 58 types, including 2 to orders, 13 to families, 14 to genera, and 29 to species, total of 58 taxa. Among them, 16 species are the first descriptions from the literatures. Certainly, many species share the same egg morphology, such as moray eels, sweepers, and cutlassfishes. For cutlassfishes , DNA barcoding can identify both Trichiurus lepturus and Trichiurus japonicus. The later species occurred in both south and north Taiwan. Regaring reproduction information, moray eels were found spawning in September, but sweepers and cutlassfishes spawn all seasons in the water off southern Taiwan. Comparing the results of specimens identification between the BOLD non-reference, BOLD reference, and fish BOLD of Taiwan demonstrates that the correct species identification of adult fishes is important. Combination of morphological observation and DNA barcoding can identify species more efficiently and assist the preparation of identification keys for fish eggs and larval fishes. Due to the incompletion of the sequence database, half of our specimens could not be assigned to the species level. We could better understand the early life history of fish in Taiwan in the future when the COI database of adult fishes and more information on their morphological characteristics of fish eggs and larvae is more complete.
| Identifer | oai:union.ndltd.org:TW/096NTU05312023 |
| Date | January 2008 |
| Creators | Jung-Hua Chang, 張榮樺 |
| Contributors | Kwang-Tsao Shao, 邵廣昭 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 115 |
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