碩士 / 靜宜大學 / 食品營養研究所 / 96 / Chlorella vulgaris is one of the popular healthy materials in natural algae, with healthy efficiency potentially relating to glycosidase (α-glucosidase, β-glucosidase and β-glucuronidase) inhibition. Therefore, this study was to investigate the isolation, preparation, characterization, and glycosidase inhibition of various fractions from Chlorella vulgaris. The samples included: hot water extract of Chlorella (WS), enzymatic hydrolyte of Chlorella hot water insoluble (WIS) degraded by both cellulase and pectinase (E1), and hot water extract of cellulase hydrolyte from freeze-dried Chlorella powder (E2), and the large (F1) and small (F3) molecular fractions of the above samples separated by ultrafiltration. The 70 % ethanol soluble of WIS (WIS-E70S) and resistant enzyme hydrolysis insoluble substance (E1-WIS, E2-WIS) were further prepared. The results of high-performance molecular size exclusion chromatography indicated that the samples of two molecular distributions were: WS-F1 (3395 kDa and 170 kDa), WS-F3 (5 kDa and 1 kDa), E1-F1 (14 kDa and 5 kDa); those of mono-distribution included: E1-F3 (6 kDa), E2-F1 (158 kDa), E2-F3 (6 kDa). The major monosaccharides was ribose for WS-F1 (49%), galactose for E1-F1 (38 %), arabinose for E1-WIS (40 %), and glucose for the others (WS-F3, E1-F3, E2-F1, E2-F3 and E2-WIS) (approximately 30~85%). The results of enzyme inhibition and enzymatic kinetics suggested that, at a concentration of 10 mg/mL, WIS-E70S exhibited the best inhibitive effect against α-glucosidase (61%), in a mixed-type noncompetitive way implied by Lineweaver-Burk plot. WS-F1 (56%) and E2-F3 (48%) showed the greatest inhibition on β-glucosidase, in competitive and mixed-noncompetitive types, respectively. And, WIS-E70S was the most effective to reduce β-glucuronidase activity (89% at 10 mg/mL) with a strongly mixed-type noncompetitive manner.
| Identifer | oai:union.ndltd.org:TW/096PU005255010 |
| Date | January 1900 |
| Creators | Jian-Gu Lin, 林建谷 |
| Contributors | Phoency Lai, 賴鳳羲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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