碩士 / 靜宜大學 / 食品營養研究所 / 96 / It was demonstrated that chlorella has the effect of antioxidation , antitumor, lowering the blood fat and reducing blood pressure. The purpose of this study was to investigate the effect of different types of chlorella on learning and memory ability and antioxidative status in senescence-accelerated mice. 3 and 6-month-old senescence-accelerated male and female mice were divided into four groups (n=15 per group) : control group, commercial mechanical chlorella powder (MP) , commercial granule chlorella powder (GP) , ball milling chlorella powder (BM) .The ideal diet of experimental group contained chlorella 2.92g/kg fed food for mice. During experimental period, body weight, food intake, aging score and open flied activity were recorded for each group of mice. Single-trail passive avoidance test and active shuttle avoidance test were performed after 12 weeks of feeding. The biochemical parameters of serum was analyzed after the mice sacrificed. The antioxidative enzyme activities such as superoxide dismutase (SOD) , catalase (CAT) , glutathione peroxidase (GPx) and MDA concentrations were estimated in the liver. Furthmore, the β-amyloid of brain was observed. The results showed that there were no significant differences in the food intake, body weight and locomotion among four groups. The aging score of experimental groups were significantly lower than the control group in 3 and 6-month-old senescence accelerated male and female mice(P<0.05). In learning and memory test, experimental groups of 3 and 6-month-old senescence accelerated male and female mice had better improvement in active shuttle avoidance test, and single-trail passive avoidance test showed that 3-month-old senescence accelerated male and female mice significantly were better than the control group (P<0.05) .The SOD and CAT activities of experimental groups of 3 and 6-month-old senescence accelerated male and female mice were increased, and the MDA concentrations in the liver significantly lower than the control group. The β-amyloid protein deposition of brain was significantly reduced in 3-month-old senescence accelerated mice (P<0.05) ,but there was not significantly different in 6-month-old senescence accelerated mice. It is concluded that the supplement of chlorella could promote the antioxidative status and improve learning and memory ability. In this study, there was no significant difference between the experimental groups, which were made from different types. And that which type of chlorella possesses the best antioxidant capacity will further investigate.
| Identifer | oai:union.ndltd.org:TW/096PU005255014 |
| Date | January 1900 |
| Creators | Huan-yi Chu, 朱桓儀 |
| Contributors | Ming-Fu Wang, 王銘富 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 111 |
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