碩士 / 淡江大學 / 生命科學研究所碩士班 / 96 / Zebrafish (Danio rerio) is a good model for large-scale food and drug toxicity tests because the following reasons. (1) They have transparent embryos that make the organogenesis are easy to observe; (2) One pair of adult fish is capable of laying 200–300 eggs per week; (3) The zebrafish genome project is almost completed. In this thesis, we design many screening methods, such as mobility test and kidney morphology for food and drug toxicity tests using sodium benzoate, caffeine, cisplatin and ruthenium complex as positive controls. Results showed that zebrafish embryos displayed lower swimming mobility after exposure to 10 ppm of sodium benzoate but had no swimming ablity after exposure to 2000 ppm of sodium benzoate. With regards to kidney toxicities, the threshold for the sodium benzoate, caffeine, cisplatin and ruthenium complex are 500, 75, 20 and 75 ppm, respectively. In conclusion, these screening methods are excellent and effective for large-scale food and drug toxicity screening, especially for nephrotoxicities.
| Identifer | oai:union.ndltd.org:TW/096TKU05105010 |
| Date | January 2008 |
| Creators | Wei-Li Chen, 陳韋利 |
| Contributors | Yau-Hung Chen, 陳曜鴻 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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