碩士 / 淡江大學 / 生命科學研究所碩士班 / 96 / Caffeine is a typical purine alkaloid that is produced in a variety of plants, including coffee, tea, kola nuts, guarana berries and cocoa beans. In medical applications, caffeine can be used as an antagonist to the adenosine receptor for regulating the contraction of blood vessels. Epidemiological studies have described the exposure of women to caffeine during pregnancy, as well as the occurrence of congenital malformations and fetal growth retardation. It might be assumed that caffeine treatment affects blood vessel formation. However, whether caffeine treatment affects vasculogenesis and/or angiogenesis during early embryonic development are unclear. Here, we used transgenic zebrafish Tg(fli1:egfp) as a model to study the caffeine-induced toxicity of vasculogenesis/angiogenesis. We soaked the embryos into different concentrations of caffeine, and found that caffeine treatment did not affect the vasculogenesis. With regard to angiogenesis, we analysed the development of dorsal longitudinal vessels in 20 somites. Results showed that caffeine-treated embryos had less dorsal longitudinal vessels compared to vehicle-control (150 ppm ~ 500 ppm 13.52±0.34 ~ 2.82±0.22；vehicle-control:19.42±0.13). On the basis of this observation, we suggested that caffeine treatment disturbs the angiogenesis of zebrafish embryos. Furthermore, quantitative polymerase chain reaction was carried out to check the expression level of cdh5, cmlc2, flt1, and nrp1a1. We found that cdh5 expression decreased to 0.36 folds, but cmlc2, flt1 and nrp1a1 increased to 4.62, 2.44 and 6.42 folds, respectively. Our conclusion is that; caffeine affected cdh5, cmlc2, flt1, and nrp1a1 genes and consequently disburbs the angiogenesis of zebrafish
larva.
| Identifer | oai:union.ndltd.org:TW/096TKU05105018 |
| Date | January 2008 |
| Creators | Chien-hung Yeh, 葉建宏 |
| Contributors | Yau-Hung Chen, 陳曜鴻 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 60 |
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