碩士 / 國立陽明大學 / 生化暨分子生物研究所 / 96 / Previous reports have shown that many congenital diseases are related to abnormal development of the adrenal gland. However, the detailed molecular mechanism controlling adrenal development is still not clear. To analyze the development of the adrenal gland, we use zebrafish because of its ease of embryo manipulation. In fish, the interrenal organ is the homologue of the adrenal gland in mammals. Interrenal precursors firstly appear as bilateral groups of cells and then migrate towards the midline, notochord. My aim is to investigate the mechanism that regulates interrenal midline fusion by performing morpholino loss-of-function experiments. At 36 hpf, interrenal precursor cells fail to fuse in smad3b morphants. Previous reports already showed the importance of Sqt and Oep in this process. Thus these results together indicate that Nodal pathway participate in interrenal midline fusion through Sqt, Oep, and Smad3b. Fish embryos depleting 刍-catenins (ctnnb1 and ctnnb2) also showed defect in interrenal precursor fusion, indicating the role of canonical Wnt pathway in interrenal maturation. Pronephro midline fusion was normal in smad3b, ctnnb1 and ctnnb2 morphants that are defective in interrenal fusion, although pronephro and interrenal precursors are adjacent to each other and converge toward the midline in parallel. Depletion of noncanonical Wnt ligands (Wnt4a, Wnt11 and Wnt11r) did not affect interrenal fusion, although the midline fusion of heart, liver and pancreas was affected. These observations indicate that the maturation processes of different organs are regulated by different mechanisms even though their precursor moving patterns look similar.
| Identifer | oai:union.ndltd.org:TW/096YM005107029 |
| Date | January 2008 |
| Creators | Hsin-Chieh Huang, 黃信傑 |
| Contributors | Bon-Chu Chung, 鍾邦柱 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 60 |
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