碩士 / 國立中正大學 / 化學工程所 / 97 / Trehalose synthase catalyzes the conversion of maltose to trehalose. Navigating NCBI web site we found that Pseudomonas putida F1 contains a trehalose synthase gene. The gene has a length of 2067 bp and encodes for trehalose synthase with 689 amino acids, corresponding to a molecular weight of 75.62KDa.
The trehalose synthase gene was amplified from chromosomal DNA of Pseudomonas putida F1 by polymerase chain reaction (PCR) with designed primers. The recombinant plasmid was constructed by inserting PCR amplified fragment into plasmid pHT08. The recombinant plasmid was transformed into E.coli BL21. The cultured recombinant E.coli cells could be induced to overexpression the target protein.
This enzyme can catalyze the formation of trehalose directly from maltose by the intramolecular rearrangement of the α-1,4 bond of maltose to the α,α-1,1 bond of the trehalose. Study on the enzyme properties revealed that optimal pH is 8.0 and the highest conversion yield could approach to 75.4% at 25℃.
| Identifer | oai:union.ndltd.org:TW/097CCU05063035 |
| Date | January 2009 |
| Creators | CHUN-YING LEE, 李俊穎 |
| Contributors | Wen-Chien Lee, 李文乾 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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