碩士 / 嘉南藥理科技大學 / 營養與保健科技研究所 / 97 / The supernatant of ethanolic extracts from leaf of Morinda citrifolia (LES) were fractionated on a preparative HPLC RP-18 column with ethanol as eluate. Based on A280, the column was able to separate LES into five fractions (I-V). Standard (whole cell) and a modified version of the comet assay (Lysed-cell comet assay, that is cell membrane removed) was employed to investigate the effect in vitro of LES and its five fractions in the subcellular environment. Results showed that LES has a significantly concentration-dependent pro-oxidant effect. Besides, some components of LES are likely to be membrane permeable and have an indirect DNA protection in whole cells mediated via interaction with membrane and / or intracellular components. The results also indicate that some components of fraction I and III (rutin-like) are membrane impermeable and have an indirect protection mechanism against oxidative stress. In addition, fraction IV is membrane permeable and directly scavenging hydrogen peroxide. Although fraction I and IV have no genotoxicity effect, fraction II and V may be played a major role for genotoxicity and / or pro-oxidant effect in the LES. In conclusion, fraction I, III and IV might contribute significantly to exogenous antioxidants which are crucial in combating oxidative stress to human blood lymphocyte.
| Identifer | oai:union.ndltd.org:TW/097CNUP5255010 |
| Date | January 2009 |
| Creators | Yi-Jung Lu, 呂怡蓉 |
| Contributors | Shih-Ying Chen, 陳 師 瑩 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
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