碩士 / 中山醫學大學 / 營養學研究所 / 97 / Deep-seawater (DSW) is defined as the sea water at depths below 200 meters. It is pure and rich in mineral contents, i.e. calcium (Ca), magnesium (Mg), and potassium (K). Decreasing hardness of DSW is necessary for a functional DSW drinking water development; meanwhile the mineral contents in DSW are also decreased. Thus, the protective effects of functional DSW drinking water on hyperlipidemia and fatty liver induced by a high-fat/cholesterol dietary habit warrant a further study.
After one week of accumulation, 48 male Syrian Golden hamsters (5-wk age) fed a high fat/cholesterol diet (12% fat/ 0.2% cholesterol) was used as the animal model which were assigned to 4 different drinking groups randomly: 1) NDW: normal distilled water; 2) DSW300: 300ppm deep-seawater drinking water; 3) DSW900: 900ppm deep-seawater drinking water; 4) DSW1500: 1500ppm deep-seawater drinking water. The experiment lasted for 6 weeks.
Lower (P<0.05) serum TC, TAG, and atherogenic index (AI), as well as hepatic TC and TAG were observed in three functional DSW-drinking-water groups when compared to those of the NDW group. The higher (P<0.05) fecal total lipid (TC and TAG) and bile acid excretions were measured in functional DSW-drinking-water groups. Meanwhile, hepatic LDLR, CYP7A1, and PPAR-α gene expressions were upregulated (P<0.05) by drinking functional DSW-drinking-water. Moreover, functional DSW-drinking-water improved (P<0.05) serum and hepatic lipoperoxide index (MDA) and antioxidant indices (TEAC and GSH), as well as lowered (P<0.05) liver functional indices (AST and ALT) which showed a cardiovascular and hepatic protection, respectively.
It was concluded that hypolipidemic effect of DSW drinking water might be due to upregulations of LDLR, CYP7A1, and PPAR-α gene expressions, and higher fecal total lipid and bile acid excretions. Functional DSW-drinking-water also improve serum and hepatic antioxidant capacities. Via this study, 900 or 1500ppm DSW drinking water is worth to develop as an ingredient of cardioprotective and hepatoprotective drinks in the future.
| Identifer | oai:union.ndltd.org:TW/097CSMU5513045 |
| Date | January 2009 |
| Creators | Yu, 王瑜 |
| Contributors | 陳億乘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 100 |
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