碩士 / 輔仁大學 / 生命科學系碩士班 / 97 / Alzheimer’s disease (AD) comprises a highest prevalence among neurodegeneration diseases. The amyloid β (Aβ) plaque detected in the brains of AD patients is generally considered resulting in apoptosis of sensor neuron cells. Besides the genetic issues, the external stress from environments may play a role in the pathogenesis of AD. In this study, we tried to establish the in vitro experimental condition of the external stress (glucose deprivation, chemical hypoxia and heat treatments) induced cell death for NT2, BV2 and SH-SY5Y cells, and investigated the corresponding role of Aβ as well. It was fond that each external stress treatments brought about apoptosis in neuron cells. However, only the chemical hypoxia and heat induced apoptosis showed a correlation of A expression in neuron cells. Intriguingly, the induced A was detected in cytosol as well as the plasma membrane of cells. Additionally, the exogenously supplied zinc ions and Tris salts reversed the apoptosis induced by external stress. Taken together, our results imply the possibility that specific external stress may induce the accumulation of A in the plasma membrane, which may form ion channels disturbing the balance of ions (calcium) and resulting in the apoptosis of neuron cells. Furthermore, when the proteomes of neuron cells were analyzed by two-dimensional electrophoresis (2-DE), a specific protein expression pattern was simultaneously observed in three cell lines under the same external stress. Therefore, merely analyzing the 2-DE patterns of samples seems sufficient to discriminate the physiological outcome of treated cells or organisms. We term the above concept as 2-DE physiognomy.
| Identifer | oai:union.ndltd.org:TW/097FJU00105016 |
| Date | January 2009 |
| Creators | Weng, Wei-Yi, 翁維誼 |
| Contributors | Chen, Han-Min, 陳翰民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 100 |
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