碩士 / 弘光科技大學 / 環境工程研究所 / 97 / Recently, it has been known for some time that heavy metal ions accumulated by aquatic organism, such as algal, bacteria and fungi. Many studies on heavy metals polluted coaters have revealed that metal could be adsorption or absorption by algal and cyanobacteria (blue-green algae). Mercury (Hg) is one of the most toxic metals commonly found in the environmental. The distribution of mercury species amount different ecosystem compartments critically influences Hg fate and bioaccumulation (could be accumulated to million times of origin concentration) in the food web. On the other hard, the Hg could be transfer by microorganism become to organic mercury, one of the toxic substances is methylmercury (MeHg). The purpose of this study is to examine the Hg bioaccumulation by measurements of Hg and MeHg distribution patterns in algae that were separted from eurtrophic reservoir. The other aims of this study is to evaluate the potential of Hg/MeHg bioaccumulated in food web, the representative selection of an indicator species for a specified eutrophic reservoir. The results showed that Microcystis aeruginosa for mercury adsorption/absorption ability is higher than Li-Yu Lake (the main algae species of diatom and chlorella). In regard to methylmercury, two types of algae on the accumulation of Hg are much higher than MeHg. Furthermore, the study for mercury and methylmercury toxicity test in animals, use of rats (Sprague-Dawley rats) to test body, dose of mercury in accordance with feeding the experimental group divided into high and low dose experimental group (Hg-5 & algae、Hg-50 & algae), experimental group of Mercury (Hg-50), experimental group of methylmercury (MeHg-25、MeHg-250), experimental group of pure algae (algae) and the blank group (blank), according to the LD50 for mercury 2.86 mg/kg (oral) and LD50 for methylmercury 30.0 mg/kg (oral), in accordance with the weight adjusted dose feeding, rats fed for a week of periods, blood taken from rats in the residual blood concentration of trace mercury at a fixed time analysis. The results showed that adsorption of mercury in the algae and methylmercury were accumulated on the blood of rats with the dose feeding, in particular, the accumulated amount of methyl mercury obvious. The results of the analysis of total mercury in the rat blood, liver, and kidney, mercury, algae of mercury the adsorption and methylmercury accumulation in the blood lower the total amount of mercury, most of the Hg was accumulated in the liver and kidney, in particular, the kidney accumulated the highest amount of total mercury. The results also showed that DNA oxidative damage in the blood DNA damage is higher than liver and kidney tissues, which the Hg-50 & Algae harm is greater than Hg-50, that the mercury adsorption by the algae after, rats may be due to metabolism of the algae on the mouse so that the mercury caused greater harm. In lipid peroxidation showed that methylmercury to rats caused a injury is higher than the mercury.
| Identifer | oai:union.ndltd.org:TW/097HKU05515007 |
| Date | January 2009 |
| Creators | Cheng chia-yu, 張家瑜 |
| Contributors | Huang winn-jung, 黃文鑑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 113 |
Page generated in 0.002 seconds