碩士 / 高雄醫學大學 / 生物醫學檢驗學研究所 / 97 / Objective: Hyper-ferrous state is the result of multiple transfusions, whereby the iron excess in such conditions may be toxic to the organism through increased oxidative stress. Therefore, we investigated the changes of oxidative stress-related biomarkers in thalassemia patients
with excessive iron.
Materials and Methods: A total of 23 a-thalassemia and 36
b-thalassemia patients from Kaohsiung Medical University Chung-Ho Memorial Hospital and 73 healthy subjects as the controls were enrolled in this study. The biochemical tests (serum iron [Fe], transferring [TRF], ferritin, total iron binding capacity [TIBC]), and the biomarkers of
oxidative stress-related tests (superoxide dismutase [SOD], glutathione peroxidase [GPx], and glutathione reductase [GRx]; reduced glutathione [GSH], oxidized glutathione [GSSG), vitamin C [Vit C], total Nitric
Oxide [Total NO], superoxide anion [O2•-], and malondialdehyde [MDA]) were measured.
Results: Activities of antioxidant enzymes (SOD, GPx and GRx); levels of Fe, MDA, and the generation of O2•- in the patients with a-thalassemia were significantly higher than those in the controls (p<0.05). Conversely, the levels of TRF, TIBC, GSH, Total GSH, and Vit C in the patients with a-thalassemia were significantly decreased compared to those in the controls (p<0.05). Activities of SOD and
levels of Fe, O2•-, Total NO, NO2-, and MDA in b-thalassemia patients were significantly higher than those in the controls (p<0.05); however, concentrations of TRF, TIBC and Vit C in the patients were significantly
lower than those in the controls (p<0.05). In addition, there were significantly higher levels of Fe, Total NO, NO2
-, and the generation of O2•- in the b-thalassemia than in the a-thalassemia. Conversely, there were significantly lower levels of TRF and TIBC, and activities of SOD,
GPx, and GRx in the b-thalassemia than in the a-thalassemia.
Conclusion: Oxidative stress is increased, especially in the patients b-thalassemia with iron ovweload.
| Identifer | oai:union.ndltd.org:TW/097KMC05114003 |
| Date | January 2009 |
| Creators | Yi-Ju Wu, 吳依儒 |
| Contributors | Li-Yu Tsai, 蔡麗玉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
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