碩士 / 高雄醫學大學 / 醫學放射技術學研究所 / 97 / Alzheimer’s disease is the most common cause of dementia of late life. A diagnosis is made primarily on clinical grounds and, to date, there is no reliable diagnostic test. The use of proteomic techniques to identify disease-specific protein biomarkers is a powerful tool for defining the prognosis of disease and gaining deep insights into disease mechanisms in which proteins play major roles.
In this study, we expect to create a detection method for specific target proteins and applications in the biomarker discovery research. To enhance our understanding of Alzheimer’s disease proteome, the plasma proteins will be identified by two-dimensional gel electrophoresis and nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry followed by peptide fragmentation patterning. In our preliminary proteomic data, seven protein markers were indicated as Alzheimer’s disease associated proteins. It suggested that those proteins, such as angiotensin converting enzyme, apolipoprotein A-IV, inter-alpha-trypsin inhibitor heavy chain H4, alpha-1-antitrypsin, activity-dependent neuroprotector homeobox protein and antithrombin-III, may offer protection for Alzheimer’s disease diagnosis. In this study, seven proteins were confirmed by ELISA or Western blotting methods. For those proteins, activity-dependent neuroprotector homeobox protein was down-regulated, and others were up-regulated in Alzheimer’s. However, the results of these studies still need to be verified by larger clinical studies.
| Identifer | oai:union.ndltd.org:TW/097KMC05605001 |
| Date | January 2009 |
| Creators | Li-Jhen Chen, 陳藜臻 |
| Contributors | Yu-Chang Tyan, 田育彰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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