碩士 / 國立中興大學 / 生命科學系所 / 97 / R-spondin (Rspo) family of secreted proteins represent a novel class of ligand capable of activating Wnt/β-catenin signaling pathway. However, homozygous knockout of rspo3 in mouse ES cells results in early embryonic fatality due to impaired formation of the labyrinthine layer of the placenta, precluding characterization of its functions during murine development. As a result, the role of rspo3 during embryonic development remains unclear. We reported here the expression pattern and the knockdown phenotypes of rspo3 gene during zebrafish embryogenesis. Transcripts of rspo3 were first detected ubiquitously in the onset of gastrulation. From the beginning of somitogenesis, the signals became to be expressed in restricted regions, including dorsal neural tube and hypochord. Using rspo3-specific morpholino, we analyzed the vasculature in Tg (fli1:egfp)y1 rspo3 morphant embryos and found that rspo3 play a pivotal role in developmental angiogenesis. The angiogenic defect in rspo3 morphants was partly attributed to the reduced expression of vegfaa. In addition, rspo3 morphants displayed defects in left-right patterning of heart. The perturbed cardiac laterality seen in embryos with reduced rspo3 expression might be due to defects in both Kupffer’s vesicle development and lefty1 expression in the embryonic midline. These data indicated that rspo3 gene is essential for the angiogenesis and establishment of cardiac asymmetry during zebrafish embryogenesis.
| Identifer | oai:union.ndltd.org:TW/097NCHU5105020 |
| Creators | Chiung-Fang Chang, 張瓊方 |
| Contributors | 蔡振寧 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
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