碩士 / 國立中興大學 / 生物產業機電工程學系所 / 97 / The purpose of this study is to develop a high density cultivation model for rotifer which is used as a design basis of the establishment of a high density cultivation system for rotifer mass culture. The designed parameters of the high density rotifer cultivation model were obtained by its environment and feed factors tests. The results of the environmental experiments have shown that the best environment conditions for rotifer cultivation were set at a temperature of 30 ℃, salinity 25ppt, pH7.5 ~ 8.5 and aeration with 0.045 vvm. In the feed factor tests, the effects of the initial density and the ESD (equivalent spherical diameter) of feeds were investigated. The three initial feed densities, 1,000, 1,500 and 2,000×104 cells/ml were selected for the cultivation tests. The results of experiments had demonstrated that a higher initial feed density obtained a greater egg ratio of the rotifer. The test with the initial feed density 2,000×104 cells/ml held the highest egg ratio and the growth rate with 0.5543 eggs/female and 0.0686 hr-1 , respectively. The density at 24 hr after inoculation was up to the initial inoculation density of 1.92 times. The experimental results had also expressed a good linear relationship between the egg ratio and the interval growth rate of the rotifer. Therefore, it will be plausible to utilize this linear relationship with egg ratio predict the trends for the growth of rotifer density in the future. During the feed factor tests, the effects using the same kind of alga with different ESD were also performed. The experimental results had shown that the ESD had significantly influenced the growth of the rotifer density, while the rotifer feeding with a larger ESD could have a better growth rate. Based on the environmental and feed experimental results, a semi-continuous culture experiment was conducted to explore the relationship with the feed density and the growth of rotifer. The experimental results had shown that as the feed consumption rate kept between 0.2~0.6×104 cells/ind., it was able to maintain an appropriate egg ratio, as well as to increase the growth of the rotifer. The highest density 6,876 ind./ml was detected after 64 hr of inoculation and the density was 8.76 times as compared with the initial inoculation density of 785 ind./ml. Ultimately, this study established a high density rotifer cultivation system. The system was constructed and combined with the parts of the microalgal reactor and the rotifer culture tank to verify the influence of the rate of feed consumption to the growth rate in the actual rotifer cultivation system. For the cultivation test of 104 hr, the average feed consumption was maintained at 0.4×104 cells/ind. The experimental results indicated that a good cultivating performance of the system with excellent rotifer growth and egg ratio was obtained. The final numbers of the rotifer were 17,355,000 individuals, and it was 2.56 times of its initial numbers 6,780,000 individuals. The average of the egg ratio was also maintained with 0.559 eggs/female. This result had demonstrated that the system with excellent capability of feed density control and high density rotifer cultivation was developed.
| Identifer | oai:union.ndltd.org:TW/097NCHU5415009 |
| Creators | Yu-An Ma, 馬聿安 |
| Contributors | 陳俊明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
Page generated in 0.0116 seconds